大鼠长期静脉输液技术

A technique for long- term intravenous infusion in rats

为了完善小动物持续经静脉用药及 TPN支持技术 ,设计一种灵巧的肝素帽旋转输液系统 ,经腔静脉置管 ,在大鼠活动不受限制时长期输液。平均时间 10 d,部分时间 >3周 ,动物均能良好耐受 ,未发生导管扭曲及液体渗漏 ,能够保证用药及热能、氮量供应 。

In order to develop a technique which provides not only a route for continuous intravenous administration of drugs,but easy access for TPN support in unrestrained rats as well,we designed a fluid infusion swivel system with implanting and maintaining venous catheter in the superior vena cava of rats,and administered a long- term intravenous infusion in unrestrained rats. The results showed that the average period for which infusion was carried out was 1 0 days,and the infusion was maintained for up to 3week in some of rats.All animals appeared to tolerate infusion catheter without obvious discomfort and minimal restriction of their motion.Kinking and leakage did not occur anymore.We conclude thatthis technique can be readily utilized and has great potential value for studying problems related to surgical nutrition and metabolism.