发热对豚鼠胆汁成分的影响

Influence of fever on biliary elements of guinea pigs

目的研究发热对豚鼠胆汁成分及胆石形成的影响．方法将豚鼠６０只分为致石饲料组和普通饲料组，再将每组分为发热组和对照组．皮下注射煮沸脱脂牛奶１ｍｇ／ｋｇ使发热组动物发热，每周１次，共４次．４５ｄ后将６０只豚鼠全部处死，其中发热组在处死前３６ｈ皮下注射松节油１ｍｌ／ｋｇ，使其在处死前处于发热状态．检查胆囊并取胆汁进行分析．结果致石饲料发热组结石发生率最高，为４００％，而致石饲料不发热组为１３３％（Ｐ＜００１）．发热组胆汁总蛋白（２５ｇ／Ｌ±１９ｇ／Ｌｖｓ１０ｇ／Ｌ±０９ｇ／Ｌ，Ａ２ｖｓＡ１）及胆红素浓度（７４３μｍｏｌ／Ｌ±６２６μｍｏｌ／Ｌｖｓ１２３μｍｏｌ／Ｌ±５５μｍｏｌ／Ｌ，Ｐ＜００５）较对照组明显升高．结论发热对豚鼠胆汁成分的影响在胆石形成中具有重要作用．

IM To study the influence of fever on biliary elements and gallstone formation in guinea pigs. METHODS Sixty guinea pigs were divided into lithogenic (group B) and normal diet (group A) groups, and each group was further divided into control and fever subgroups. The animals in the fever groups were injected subcutaneously with boiled defat milk 1ml/kg, once a week for 4 times to run fever. After 45 days, all the animals were killed. Among them the guinea pigs in the fever groups were injected subcutaneously with turpentine 1ml/kg 36 hours before they were killed, and kept in fever condition. Their gallbladder lumens were examined and bile samples were analyzed. RESULTS The gallstone incidence of the fever group fed with lithogenic diet was the highest, accounting for 40% (6/15). All the animals in fever group had significantly higher concentration of total bile protein and bilirubin than that of the control groups. CONCLUSION The effect of fever on biliary elements plays an important role in the gallstone formation in guinea pigs.