幽门螺杆菌阳性胃粘膜病变AgNOR和rasp21的表达

AgNOR and rasp21 expression in gastric mucosal lesions with Helicobacter pylori infection

目的研究幽门螺杆菌阳性（Ｈｐ＋）不同胃粘膜病变的ＡｇＮＯＲ数量及ｒａｓｐ２１阳性表达率，探讨其生物学行为及Ｈｐ在此过程中可能的作用．方法经内窥镜病理检查证实胃粘膜病变（慢性浅表性胃炎、慢性萎缩性胃炎、肠上皮化生、异型增生、胃癌）共计２７８例．通过Ｈｐ检测（ＣＬＯｔｅｓｔ结合ＷａｒｔｈｉｎＳｔａｒｙ染色）证实其中１４６例阳性，１３２例阴性．分别对其粘膜标本作了ＡｇＮＯＲ定量及ｒａｓｐ２１表达的研究，比较不同胃粘膜病变中Ｈｐ阳性和阴性组间ＡｇＮＯＲ数目和ｒａｓｐ２１阳性表达率．结果除慢性浅表性胃炎外各病变中Ｈｐ＋组的ＡｇＮＯＲ数量均显著高于Ｈｐ－组（Ｐ＜００５或Ｐ＜００１）；除慢性浅表性胃炎及慢性萎缩性胃炎外各病变中Ｈｐ＋组的ｒａｓｐ２１阳性表达率均显著高于Ｈｐ－组（Ｐ＜００５）．结论Ｈｐ＋胃粘膜病变具有更多的肿瘤生物学行为，该菌可能刺激胃上皮细胞的过度增殖而启动恶性变．

IM To study the number of AgNOR and rasp21 expression in different gastric mucosal lesions with Helicobacter pylori (Hp) infection to evaluate their biological behaviour and possible mechanism of Hp. METHODS Hp (using CLO test combined with WathinStarry staining), AgNOR (silver colloid technique) and rasp21 (monoclonal antibody and immunohistochemical staining—ABC method) were detected in 278 patients with endoscopically and pathologically confirmed gastric mucosal lesions, including chronic superficial gastritis (CSG), chronic atrophic gastritis (CAG), intestinal metaplasia (IM), dysplasia (Dys), and gastric cancer (GC). Among them, 146 cases were Hp positive, and 132 cases Hp negative. RESULTS The mean number of AgNOR in Hp positive group was significantly higher than that in Hp negative group in the gastric mucosal lesions except for CSG (P<005 or P<001). The positive rate of rasp21 expression in Hp positive group was also significantly higher than that in Hp negative group in gastric mucosal lesions except for CSG and CAG (P<005).CONCLUSION Hp+ gastric mucosal lesions have more biological behaviour of tumors. Hp may act as a promoter to activate ras gene and to stimulate cell over proliferation.