离体肺气体容积和肺密度在肺气肿小鼠模型中的变化

Changes of excised lung gas volume and lung density in murine emphysema model

目的研究以猪胰弹性蛋白酶(PPE)诱导小鼠肺气肿模型的离体肺气体容积(ELGV)和肺组织的密度变化规律,探讨ELGV和肺密度与肺气肿的相关性。方法30只雄性C57BL/6小鼠随机均分为对照组、PPE 3d组和PPE 7d组,气管内滴注PPE,建立肺气肿模型。以密度测量仪测量ELGV和肺组织密度,同时进行肺组织病理学观察及图像半定量分析。结果与对照组相比,PPE 3d组和PPE 7d组肺湿重的差异均无统计学意义(P值均>0.05)。对照组、PPE 3d组和PPE 7d组的ELGV分别为(0.217±0.011)、(0.271±0.016)和(0.420±0.034)cm^3,PPE 3d和PPE 7d组显著高于对照组(P值均<0.01),PPE 7d组又显著高于PPE 3d组(P<0.01)。对照组、PPE 3d组和PPE 7d组的肺组织密度分别为(0.606±0.016)、(0.541±0.033)和(0.368±0.032)g/cm^3,PPE 3d和PPE 7d组显著低于对照组(P值均<0.01),PPE 7d组又显著低于PPE 3d组(P<0.01)。结论ELGV和肺组织密度与肺气肿的形态学改变密切相关,可以作为监测和评价肺气肿形态学改变的可行性指标。

Objective To study the changes of the excised lung gas volume （ELGV） and the lung density in murine emphysema model induced by porcine pancreatic elastase （PPE） intratracheally, so as to further clarify the relationship of ELGV, lung density with emphysema. Methods Thirty male C57BL/6 mice were randomly divided into 3 groups： PPE 3 d group, PPE 7 d group and the control group. Murine emphysema model was established by intratracheal infusion of PE. A density determination kit was used to determine the ELGV and lung density of each mouse. Meanwhile, the pathological examination and semi-quantitative analysis were performed. Results There was no significant difference in the wet weight of lungs between the 3 groups. The ELGV values of the PPE groups were significantly higher than that of the control group （[0. 217 ± 0. 011] cm3） , and that of the PPE 7 d group （[0. 420±0. 034] cm3） was significantly higher than that of the PPE 3 d group （[0. 271 ± 0. 016] cm3） ; meanwhile, the lung density of the PPE groups were significantly lower than that of the control group （[0. 606 ± 0. 016] g/cm3）, and that of the PPE 7 d group （[0. 368 ± 0. 032] g/cm3 ） was significantly lower than that of the PPE 3d group （[0. 541± 0. 033] g/cm3 , P〈 0.01）. Conclusion Both ELGV and lung density are closely associated with the lung morphological alteration during emphysema, and they might serve as the potential indicators of emphysema.