白鹃梅离体快繁技术的研究

Study on Rapid Propagation Technique of Exochorda racemosa in vitro

[目的]探讨白鹃梅离体快速繁殖技术。[方法]以MS为基本培养基,附加不同种类、不同浓度细胞分裂素（6-BA、KT）和生长素（IBA、NAA）,诱导离体白鹃梅快速繁殖。[结果]结果表明,较低浓度的细胞分裂素6-BA、KT和生长素IBA混合使用,有利于诱导芽增殖,其中以MS＋0.3 mg/L IBA＋0.5 mg/L 6-BA配方最佳。用1/2 MS附加0.1～0.3 mg/L NAAI、BA均能诱导生根,其中以1/2 MS＋0.3 mg/LNAA配方最佳。

[Objective] This research aimed to explore the rapid propagation technique of Exochorda racemosa in vitro.[Method] E.racemosa were cultured in the MS medium which contained different kinds and densities cytokinin（6-BA,KT） and auxin（NAA,IBA） to induce the rapid propagation.[Result] The results showed that mixed use of lower concentration of cytokinin（6-BA,KT）and auxin（IBA）were helpful for the induction of bud propagation,and the optimum formula was MS＋IBA 0.3 mg/L＋6-BA 0.5 mg/L.Both NAA and IBA with 0.1-0.3 mg/L could induce the root when it was added into 1/2 MS medium and the optimum medium was 1/2 MS＋0.3 mg/L NAA.