土壤铝存在形式对马铃薯抵抗侵染软腐病菌的影响

Influences of Existing Forms of Aluminum in Soil on the Resistance of Potato to Infection of Soft Rot Bacteria

[目的]从土壤化学角度探讨西北地区适合种植马铃薯的病理原因。[方法]通过模拟酸性及碱性土壤中铝的2种形式氯化铝(Al3+)和胶体铝(Al(OH)3),研究马铃薯块茎受软腐病菌侵染时土壤胶体态的铝对块茎过氧化氢积累及病程蛋白表达的影响。[结果]土壤铝的溶出直接导致马铃薯块茎切片细胞的死亡。马铃薯感染软腐病菌过程中,Al3+处理加速了马铃薯的感病,而Al(OH)3则延缓了马铃薯的感病。Al3+处理在加速马铃薯细胞死亡的同时产生大量的H2O2,而Al(OH)3在产生较高H2O2积累的同时并未造成明显的马铃薯细胞死亡。SDS-PAGE电泳分析表明,Al(OH)3处理的马铃薯明显不同于Al3+及空白对照。[结论]马铃薯块茎在感病过程中受土壤铝溶出率及铝形态变化的双重影响。

[Objective] The study aimed to discuss the pathological cause of northwest region suitable for planting potato in soil chemistry.[Method] Through simulating the 2 existing forms of aluminium chloride（Al3＋） and colloidal aluminium（Al（OH）3） in acid and alkali soils,the influences of Al（OH）3 on the hydrogen peroxide accumulation and the expression of pathogenesis-related proteins in potato tuber when it was infected by soft rot bacteria were studied.[Result] The dissolution of aluminium in soil directly led to the death of slice cells of potato tuber.During the course of potato infecting soft rot bacteria,Al3＋ accelerated the disease infection of potato and Al（OH）3 delayed it.Al3＋ accelerated the death of potato cells and produced much H2O2,but Al（OH）3 caused higher H2O2 accumulation and did not cause the significant death of potato cells.SDS-PAGE electrophoresis analysis showed that the potato treated by Al（OH）3 was significantly different from that treated by Al3＋ and CK.[Conclusion] There was dual influenc e of dissolution rate of aluminium in soil and its form change on potato tuber during disease infection.