细胞周期素H及细胞周期素依赖性激酶-7在皮肤血管瘤组织中的表达及意义

Expression and clinical significance of cyclin H and CDK7 in human hemangiomas

目的探讨细胞周期素H（cyclinH）、细胞周期素依赖性激酶-7（CDK-7）在人皮肤血管瘤组织中的表达及临床意义。方法采用免疫组织化学方法分别检测皮肤血管瘤组织中增生期、退化期及正常皮肤组织中cyclinH和CDK-7蛋白的表达，并结合第Ⅷ因子相关抗原的免疫组织化学染色证实表达cyclinH和CDK-7蛋白的细胞是血管内皮细胞。利用计算机图像分析技术测量不同时期血管瘤组织和正常皮肤组织cyclinH和CDK-7表达的平均吸光度A值和平均阳性面积。结果增生期血管瘤内皮细胞cyclinH和CDK-7的表达水平明显高于退化期及正常皮肤组织，差异有统计学意义（P〈0．01）；退化期与正常皮肤组织血管内皮细胞cyclinH和CDK-7表达水平差异无统计学意义（P〉0．05）。结论CDK7和cyclinH在增生期血管瘤组织中促使内皮细胞大量增生，使血管瘤不断生长，提示CDK7和cyclinH在血管瘤的发生、发展中起了重要作用。

Objective To investigate the expression and clinical significance of cyclin H and cyclindependent kinase 7（CDK7） in human hemangiomas. Methods Immunohistochemistry technique was used to measure the expression of cyclin H and CDK7 proteins in proliferative, involuting hemangiomas and normal skin tissues. Immunohistochemical technique for factor V^-related antigen was used to prove that the cells which expressed cyclin H and CDK7 were endothelial cells. Average optical density and positive area of the expression of cyclin H and CDK7 proteins in proliferative, involuting hemangiomas and normal skin tissues were measured by image analysis （HPIAS-1000）. Results The expression of cyclin H and CDK7 protein in proliferating hemangiomas was significantly higher than that in involuting hemangiomas and normal skin tissues （ P 〈 0.01 ） . But no significant difference was found in the expression of cyclin H and CDK7 protein between involuting hemangiomas and normal skin tissues （ P 〉 0.05 ）. Conclusions cyclin H and CDK7 may play an important role in the generation and development of human hemangiomas.