oxLDL和大豆异黄酮对滋养细胞LOX-1表达的调控及意义

The Regulation of oxLDL and Soyboan Isoflavones on Expression of LOX-1 in Trophoblast Cells and its Significance

目的通过检测氧化修饰低密度脂蛋白(oxLDL)和大豆异黄酮(SI)对绒癌细胞株JEG-3细胞血凝素样氧化修饰低密度脂蛋白受体1(LOX-1)表达的调控,来探讨SI对滋养细胞的抗氧化作用,为其应用于子痫前期的防治提供一定的实验基础。方法以具有妊娠早期细胞滋养细胞特性的绒癌细胞株JEG-3细胞为研究对象,用细胞免疫化学、Westernblot、RT-PCR方法检测oxLDL和不同浓度的SI对JEG-3中LOX-1表达的调控。结果通过细胞免疫化学技术检测到LOX-1在JEG-3细胞浆及细胞膜中均有表达。oxLDL可诱导JEG-3细胞LOX-1mRNA和蛋白的表达;在oxLDL作用基础上,加入不同终浓度的SI共培养24h后,又可剂量依赖的逆转上述作用。结论推测SI在JEG-3细胞可能通过受体途径LOX-1对抗oxLDL的氧化损伤作用。

Objective To discuss the anti-oxidized effect of soybean isoflavones by determining the influence of oxLDL and soybean isoflavones on the expression of LOX-1 in trophoblast cells, so as to supply the experimental basis for the use of soybean isoflavones for the prevention and treatment of preeclampsia. Methods Chofioepithelioma cloned strain （JEG-3） with the characteristic of trophoblast cells was cultured in vitro,and the regulation of LOX-1 expression in trophoblast cells when added with oxLDL and different concentrations of soybean isoflavones was measured by cell immunochemistry, RT-PCR and Western-blot. Results The expression of LOX-1 was detected in cytolymph and cellular membrane of JEG-3 cells by cyto-immunochemistry, oxLDL could induce the expression of LOX-1 mRNA and protein in JEG3, which could be reversed close-dependently after SI was added at different concentrations and cultured for 24 hours. Conclusion It can be inferred that soybean isoflavones may antagonize the oxidized effect of oxLDL in JEG-3 through the receptor path.