摘要
目的探讨CCNH基因启动子区-1SNP位点碱基变异对CCNH基因转录活性的影响。方法通过PCR和定点突变技术,构建了含CCNH基因基础启动子及-1G突变启动子,用双荧光素酶报告系统观察此SNP对CCNH基因启动子转录活性的影响。结果在AD293细胞中,-1G突变启动子活性比CCNH基础启动子活性低26%(P<0.05)。结论-1T→G碱基变异可显著降低CCNH基因启动子转录活性。
Objective To study the effect of-1 site single nucleotide polymorphism （SNP） on CCNH gene promoter transcription activity. Methods PCR and site-directed mutagenesis technology were used to construct CCNH basic promoter and -1G mutate promoter. Dual-Luciferase Reporter assay system was used to detect the transcription activity of constructed promoter. Results In AD293 cells, the activity of -1G mutate type promoter was significantly lower than that of wild type-1T promoter（ P 〈 0. 05 ）. Conclusion SNP-1 T→G base variation can decrease the transcription activity of CCNH gene promoter.
出处
《基础医学与临床》
CSCD
北大核心
2008年第7期734-737,共4页
Basic and Clinical Medicine
基金
上海教育发展基金会晨光计划
南通大学校级课题(07Z029)
