基质细胞衍生因子-1及受体CXCR4在阴道念珠菌病小鼠模型中的表达

Expression of stroma-derived factor 1 and its receptor CXCR4 in a murine model of vaginal candidi- asis

目的检测基质细胞衍生因子-1（SDF—1）及其受体CXCR4在阴道念珠菌病小鼠模型阴道组织及腰引流淋巴结中的表达情况。方法采用雌激素化阴道念珠菌病小鼠模型，雌性昆明小鼠（80只）随机分四组，A组（雌激素处理感染组）、B组（雌激素处理未感染组）、C组（未用雌激素处理感染组）、正常对照组（5只）。前三组又再分为接种白念珠菌后第2、4、7、14和21天组（各5只）。接种后不同时间点随机取小鼠阴道组织及腰淋巴结，以RT-PCR及免疫组化SABC法检测SDF—1及CXCR4mRNA及蛋白水平的表达。结果（1）RT-PCR：与正常小鼠相比，雌激素（B组）对SDF—1及CXCR4mRNA表达无影响。①小鼠阴道组织：与B组相比，A组小鼠接种后各时间点均见SDF—1mRNA表达明显增加（F=114．87，P〈0．01），第14天CXCR4mRNA明显增加（t=15．61，P〈0．01）；与正常小鼠相比，C组小鼠接种后第2，4，7天，SDF-1mRNA明显增加；第4～21天CXCR4mRNA均明显增加。②腰淋巴结：与B组相比，A组小鼠接种后第2天和7～21天SDF-1mRNA明显增加（F=865．62，P〈0．01），CXCR4mRNA于各时间点表达均增加（F=161．30，P〈0．01）；与正常小鼠相比，C组小鼠接种后SDF—1mRNA表达无明显改变（P〉0．05）：CXCR4mRNA于第7天和14天明显增加（P〈0．05）。（2）免疫组化：A组小鼠阴道黏膜中SDF—1及CXCR4表达在接种后第2天即开始增加，第7天时达高峰，第14天和21天开始下降，但均明显高于对照组（P〈0．05）。结论感染组小鼠阴道黏膜及腰淋巴结中SDF—1及CXCR4的mRNA及蛋白水平均明显升高。

Objective To examine the expressions ofstroma-derived factor 1 （SDF-1） and its receptor CXCR4 in murine vaginal tissue and lumbar lymph nodes during an experimental Candida albicans vaginal infection. Methods A murine model of vaginal candidiasis was established. Eighty female mice were ran- domly divided into 4 groups, 25 in group A （estrogen-treated mice inoculated with referrence C. albicans strain）, 25 in group B （estrogen-treated mice inoculated with phosphate buffered solution）, 25 in group C （untreated mice infected with C. albicans） and 5 untreated mice flee of infection in the control group. The first 3 groups were equally divided into 5 subgroups, including 2-, 4-, 7-, 14- and 21-day groups. The mRNA and protein expression of SDF-I and CXCR4 in the murine vaginal tissue and draining lumbar lymph nodes was assessed by RT-PCR and immunohistochemistry, respectively, 2, 4, 7, 14 and 21 days aider the inocula- tion of C. albicans. Results No significant difference was observed in the mRNA expression of SDF-1 or CXCR4 between group B and the control group. Compared with group B, the mRNA level of SDF-1 in group A increased both in vaginal tissue from day 2 to 21 （F = 114.87, P 〈 0.01 ） and in lumbar lymph nodes on day 2, 7, 14 and 21; increased mRNA expression of CXCR4 was observed in vaginal tissue on day 14 （t= 15.61, P〈 0.01） and lumbar lymph nodes from day 2 to 21 （F= 161.30, P〈 0.01） in group A. In group C, the mRNA expression of SDF-1 significantly increased in vaginal tissue on day 2, 4 and 7 but remained unchanged in lumbar lymph tissues through the test period （P 〉 0.05 ）, and an significant increase was seen in mRNA expression of CXCR4 in vaginal tissue from day 4 to 21 and in lumbar lymph tissues on day 7 and 14 in comparison with the control group expression levels of SDF-1 and CXCR4 in vaginal （all P 〈 0.05）. As immunohistochemistry showed, the tissue of group A commenced to increase on day 2,reached a peak on day 7, then decreased on day 14 and 21 following the infeciton （P 〈 0.05 ）, but they were consistently higher than those of the control group through the study period. Conclusion An increase in mRNA and protein expressions of SDF-I and CXCR4 is noticed in vaginal tissue and lumbar lymph nodes of C. albicans-infected mice.