摘要
目的探讨1%联苯苄唑乳膏体内外抗真菌活性及体内抗炎作用。方法采用豚鼠癣病模型、真菌体外药敏试验及小鼠二甲苯耳廓肿胀法评价1%联苯苄唑乳膏体内外抗真菌活性及体内抗炎作用。结果1%联苯苄唑乳膏能有效治愈豚鼠须毛癣菌感染;体外抗皮肤癣菌和念珠菌最小抑菌浓度范围为0.016~0.5mg/L,最小杀菌浓度范围为0.063~2.0mg/L;该药能显著抑制二甲苯所致的小鼠耳廓肿胀。结论1%联苯苄唑乳膏具有明显抗皮肤癣菌、抗念珠菌和抗炎作用。
Objective To study the in vivo and in vitro antifungal activity as well as in vivo antiinflammatory effect of bifonazole 1% cream. Methods Guinea pig model of dermatophyte infection was established with the inoculation of Tnchonphyton mentagrophyton on the back of guinea pigs. Following the establishment of the infection, bifonazole I% cream was topically applied to the lesions once a day until clinical and mycological cure was achieved. Clinical and mycological examination was performed daily until 2 weeks after the achievement of clinical and mycological cure. Microdilution method was utilized to test the antifungal activity of bifonazole 1% cream. The minimal inhibitory concentration (MIC) and minimal fungi- cidal concentration (MFC) were determined. The swelling of mouse auricle was induced by xylene and treated with bifonazole 1% cream. Terbinafine 1% cream served as the control drug. Results Bifonazole 1% cream and terbinafine 1% cream successfully controlled the dermatophyte infection in guinea pigs. As susceptibility test showed, the in vitro MIC of bifonazole cream ranged from 0.016 to 0.5 rag/L, MFC ranged from 0.063 to 2.0 mg/L for dermatophyte and Candida strains. Furthermore, bifonazole I% cream significantly inhibited the swelling of mouse auricle induced by xylene. Conclusion Bifonazole 1% cream shows potent anti-dermatophyte, anti-candida, anti-inflammation effects.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2008年第8期522-525,共4页
Chinese Journal of Dermatology