中国北方汉族人乙二醛酶I基因Ala111Glu多态性与糖尿病并发冠心病的关系研究

Association of Glyoxalase I Gene Ala111Glu Polymorphism and Type2 Diabetes Mellitus with Coronary Heart Disease in Han Nationality from Northern China

目的探讨中国北方地区汉族人乙二醛酶I（GLO—I）基因AlalllGlu多态性与糖尿病并发冠心病的关系。方法应用聚合酶链反应限制性片段长度多态性（PCR—RFLP）技术，检测了161名对照组、99例糖尿病组和71例糖尿病并发冠心病组GLO-I基因AlalllGlu多态性的基因型和等位基因频率分布。分析基因多态性对糖化血红蛋白（HbAlc）、血糖、血脂水平的影响。结果3组研究对象的GLO—I基因AlalllGlu多态性基因型和等位基因频率分布差异无显著意义，不同基因型亚组间HbAlC、血糖、血脂水平无明显差别。Logistic回归分析显示，年龄、HbAlc是糖尿病并发冠心病的危险因素，HDL—C则是糖尿病并发冠心痛的保护因素（β=-2．708，Exp（β）=0．067，95％CI=0．009～0．488，P=0．008）。结论GLO-I基因AlalllGlu多态性与糖尿病并发冠心病无明显关联性，不是中国北方汉族人糖尿病并发冠心病发病的独立危险因素。

Objective To investigete the association between glyoxalase I（GLO-I） gene AlalllGlu polymorphism and type 2 diabetes mellitus （DM） with coronary heart disease （CHD） in Han nationality from northern China. Methods Genotypes of AlalllGlu polymorphisms of glyoxalase I gene were analyzed by using PCR-RFLP in 161 unrelated healthy individuals,99 patients with evidence of type 2 DM,and 71 of DM complicated with CHD. The relationship of gene polymorphisms of （GLO-I） and levels of HbAlc,serum lipids,and blood sugar was also studied. Results Neither the frequencies of genotypes nor frequencies of alleles of GLO-I gene AlalllGlu polymorphisms were statistically different among DM patients,DM ＋CHD patients and controls （P〉 0. 05）. No relationship between gender, family history of HBP, BMI and （GLO-I） gene polymorphisms was found. Logistic regression analysis showed that age and HbAlc was the risk factor,but HDL-C was a protection factor （β= - 2. 708,Exp（β）=0.067,95%CI=0.009-0.488,P=0,008）for type 2 （DM） with （CHD）. Conclusion The AlalllGlu polymorphisms of GLO-I gene might not the major genetic risk factors of type 2 （DM） with （CHD） in Han nationality.