TP与ALB在不同检测系统测定结果的比对研究

Comparabilily Study on Results of TP and ALB with Different Detecting Systems

目的通过对总蛋白(TP)、清蛋白(ALB)在4个不同检测系统的对比分析,探讨不同检测系统同一项目检测结果的可比性。方法按照NCCLS的EP9-A2文件要求,以Vitros950干式化学分析法为参比方法(X),以Olympus AU640,2台Dimension Xpand-HM生化分析仪分析为待比方法(Y),用患者新鲜血清测定TP,ALB,计算相关系数及直线回归方程,以CLIA’88规定的室间质量评价允许误差范围的1/2为标准,在不同医学决定水平判断不同检测系统的偏差临床是否可以接受。结果4个检测系统测定同一检测项目相关性均良好(r>0.975);待比方法(Y1,Y2和Y3)与参比方法的结果的预期偏差在医学决定水平临床均可接受。结论不同仪器、不同测定方法测定同一检测项目时,要进行对比分析,以保证检验结果的准确、一致。

Objective The comparability of serum total protein and albumin results of four different biochemical detecting systems were studied through bias estimation and method comparison. Methods Following NCCLS document EP9-A2,detecting system including vitros 950 biochemical analyzer as comparison method,the rest three different biochemical detecting system as laboratory method and were used respectively to TP and ALE,then to obtain the correlation coefficient and the linear equation. Then the coefficient and the equation were used to evaluate the system bias between laboratory method and comparison method. Judged the comparability of different investigate systems according to the half of CLIA＇88 standard. Results The correlation between systems was good（r〉0. 975） ;the results of TP,ALB of systems 1,2 and 3 were acceptable to the comparison method. Conclusion If the same test are determined by more than two systems,it is necessary to do method comparison in order to insure the comparability ,accurate and consistent.