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SDF 1α对大鼠骨髓源性内皮祖细胞克隆形成能力及增殖的影响 被引量：7

Effects of stromal cell-derived factor-1α on proliferation and clone formation of rat bone marrow derived endothelial rrogenitor Cells
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摘要 目的观察基质细胞衍生因子-1α(SDF-1α)对体外培养的大鼠骨髓源性内皮祖细胞(EPCs)动员和增殖的影响。方法微孔法获取大鼠骨髓EPCs并采用荧光显微镜和流式细胞术鉴定EPCs特异性标记物。不同浓度SDF-1α处理EPCs后,采用细胞培养、MTT检测EPCs的克隆形成、细胞增殖。结果通过MTT法检测,对照组与(1、10、100μg/L)SDF-1α处理组的OD值分别为0.311±0.054、0.587±0.072、0.813±0.056、1.029±0.078,通过统计学方法分析,对照组与SDF-1α处理组比较差异均有统计学意义(n=5,P<0.05);100μg/LSDF-1α处理组次级内皮祖细胞集落单位数目是对照组近3倍(4.67±1.577比14.33±3.055,n=5,P<0.01)。结论SDF-1α剂量依赖性地促进EPCs增殖,并显著增强EPCs克隆形成能力。 Objective To investigate the role of stromal cell-derived factor-1α （SDF-1α）to proliferation and clone formation on endothelial progenitor cells（EPC）. Methods Bone marrow derived EPC were indfined by immunofluorescence staining and flow cytometry. Treated with different concentrations of SDF-1α, the property of proliferation was detected by MMT and clone formation was detected with optical microscopy. Results SDF-1α promoted EPC proliferation and clone formation with concentration-dependent manner. Conclusion SDF-1α can improve proliferation of EPC.
作者 夏艺萍 危当恒 童中艺 吕运成 姚峰 周晓峰 田永凤 姜志胜 王佐
机构地区 湖南省衡阳市南华大学心血管病研究所/动脉硬化学湖南省重点实验室/南华大学护理学院
出处 《中国实用医药》 2008年第21期3-5,共3页 China Practical Medicine
基金 中国博士后基金(项目编号:2005038472) 湖南省自然科学基金(项目编号:07jj3034) 湖南省教育厅课题(项目编号:07C617) 湖南省自然科学基金(项目编号:06jj5051)
关键词 基质细胞衍生因子-1Α 内皮祖细胞 增殖 Stromal Cell-Derived Factor-1α Endothelial Progenitor Cells Proliferation
分类号 R329 [医药卫生—人体解剖和组织胚胎学]
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参考文献20

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二级参考文献14

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共引文献9

同被引文献86

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中国实用医药
2008年 第21期

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