摘要
目的建立尼扎替丁注射液中尼扎替丁的含量测定方法。方法采用反相高效液相色谱法（RP-HPLC）,Hypersil ODS C18色谱柱;乙腈-0.1mol/L的醋酸铵溶液（15：85,每100ml中加0.6ml的三乙胺,用醋酸调节pH至8.5）为流动相;检测波长为242nm;流速：1.0ml/min。结果尼扎替丁在2.5～20.0μg/ml范围内具有良好的线性关系,平均加样回收率为98.67%,RSD为1.03%。结论本方法准确可靠,重现性好,可用于尼扎替丁注射液中尼扎替丁的含量测定。
Objective To establish a method for determining nizatidine in nizatidine injection by RPHPLC. Methods The separation was performed on Hypersil ODS C18 column,using acetonitrile-0. 1 mol/Lammonium acetate soluttion （ 15 ： 85 ） as mobile phase, the detection wavelengh of nizatidine was at 242 nm ; the flow rate was at 1.0 ml/min. Results The linear range of nizatidine was 2. 5-20. 0 μg/ml, the average recovery of nizatidine was 100. 13% （ RSD = 1.29% ）. Conclusion This method is found to be accurate ,reliable and have good reproducibilities. It can be used for the determination of nizatidine in nizatidine injection.
出处
《中国实用医药》
2008年第21期138-139,共2页
China Practical Medicine
