HPLC测定复方维生素注射液中维生素A、E、K_1的含量

Determination content of compound vitamins A、E、K_1 injection by high performance liquid chromatography

目的探讨高效液相色谱法（high performance liquid chromatography,HPLC）测定复方维生素注射液A、E、K1含量的可行性。方法采用HPLC法,C18色谱柱（150mm×46mm,5μm）,流动相：甲醇-酒精（80∶20）,检测波长270nm。结果维生素A棕榈酸酯在125～125.5μg/ml之间线性良好（r=0.9999）,平均回收率为99.38%,RSD为1.36%;维生素E在125～125.5μg/ml之间线性良好（r=0.9999）,平均回收率为95.87%,RSD为0.95%。维生素K1在16.5～166.5μg/m之间线性良好（r=0.9998）,平均回收率为98.08%,RSD为1.57%。结论HPLC法测定复方维生素注射液维生素A、E、K1的含量专属性强,灵敏度高,准确性好。

Objective To determine the contents in compound vitamins A,E,K1 injection by high performance liquid chromatography（HPLC）. Methods HPLC was used C18 column（150 mm ×46 mm,5 μm）was selected,the mobile phase was methanol-alcohol（80： 20）. Detection was performed by UV with wavelength of 270 nm. Results A linearity of retinyl palmitate was obtained from 125 to 125.5 μg/ml with good correlation（ r =0. 999 9）. The average recovery of retinyl palmitate was 99. 38% ,and RSD Was 1.36%. A linearity of vitamin E was obtained from 125 to 1251 μg/ml with good correlation （r = 0. 999 8）. The average recovery of vitamin E was 98.87% ,and RSD was 0. 95%. A linearity of vitamin K1 was obtained from 16. 5 to 166.5 μg/ml with good correlation （r = 0. 999）. The average recovery of vitamin K1 was 98.08 % , and RSD was 1.57%. Conclusion The RP-HPLC is sensitive and accurate in determining the contents in compound vitamin injection.