摘要
采用直接法制备传代细胞染色体标本、常规G iem sa染色、显微镜下计数中期分裂相染色体数目的方法对7种鳞翅目昆虫细胞系进行了染色体分析,得出了各种细胞系染色体分析所需要的秋水仙素浓度与处理时间以及低渗浓度和处理时间的范围;7种鳞翅目细胞系染色体均表现出典型的鳞翅目昆虫传代细胞的核型特征:染色体数目众多,异倍化严重,多为弥散型的着丝粒的短杆状、颗粒状、点状或圆球状。
The chromosomes of 7 insect cell lines from Lepidoptera were analysed. The results showed that the favorable treated concentration and time of colchicines solution and hypotonic KCI solution for each cell line, and the chromosomes of the 7 Lepidoptera cells displayed the typical karyotype characteristics of Lepidoptera cell lines ： these cells had a great variation in chromosome number, and had deeply heteroploid , chromosomes with diffuse centeromeres were short pole-like, pellet-like and sphere-like.
出处
《林业科学研究》
CSCD
北大核心
2008年第4期493-499,共7页
Forest Research
基金
国家林业局“948”项目(编号:2002-52)
林业公益性行业科研专项(编号:4-38)
关键词
鳞翅目
细胞系
染色体
核型
异倍化
Lepidoptera
cell lines
chromosome
karyotype
heteroploid
