五倍子化学成分研究

Chemical constituents of Galla Chinensis

目的研究五倍子石油醚和氯仿部位化学成分。方法利用硅胶柱色谱及薄层色谱等进行分离纯化，并通过^1H—NMR、^13C—NMR、ESI-MS等波谱技术进行结构鉴定。用二倍稀释法测定了化合物I对尖孢镰刀菌的抗菌活性。结果共分离得到10个化合物，分别为2-羟基-6-十五烷基苯甲酸（I）、白果酚（Ⅱ）、棕榈酸-1，3-二甘油酯（Ⅲ）、β-谷甾醇（Ⅳ）、正二十五烷（Ⅴ）、4-羟基-3-甲氧基-苯甲酸（Ⅵ）、棕榈酸（Ⅵ）、月桂酸（Ⅷ）、肉豆蔻酸（IX）及没食子酸（X）。结论化合物I～Ⅵ为首次从五倍子中分离得到。化合物I对尖孢镰刀菌显示中等的抗菌活性。

Objective To study the chemical constituents of the petroleum and chloroform extracts of Galla Chinensis. Methods Using chromatography on silica gel column and thin-layer chromatography to isolate and purify the compounds ; using spectroscopy methods to elucidate their structures; using double dilution to screen antibacterial activities on Fusariurn oxysporurn. Results Ten compounds were isolated and identified as 2-hydroxy-6-pentadecyl benzoic acid （ I ）, 4-（pentadec-8-enyl） phenol （ Ⅱ ）, plamitic acid-l, 3-dipropyl ester （Ⅲ）,β-sitosterol （IV）, pentacosane （V）, 4-hydroxy-3-methoxy-benzoic acid （VI）, plamitic acid （Ⅶ）, lauric acid （Ⅷ）, myristic acid （Ⅸ）, and gallic acid （X）. Conclusion Compounds I -- Ⅵ are obtained from Galla Chinensis for the first time. Compound I shows moderate inhibitory activity on F. oxysporum.