异穿心莲内酯-β-环糊精包合作用的研究

Inclusion effect of isoandrographolide-β-cyclodextrin

目的利用紫外分光光度法、红外分光光度法及核磁共振法研究β-环糊精对异穿心莲内酯的包合作用。方法采用微波辅助饱和水溶液法制备异穿心莲内酯-β-环糊精包合物,紫外分光光度法测定包合物的包合常数,并确定异穿心莲内酯-β-环糊精的包合比例,红外分光光度法和核磁共振法确证包合物的形成且验证包合比例。结果异穿心莲内酯与β-环糊精形成了包合物,包合常数为140.845 L/mol,包合比例为1∶1。结论β-环糊精可以包合异穿心莲内酯,且包合效果较好。

Objective To study the inclusion effect of isoandrographolide-β-cyclodextrin by method of UV, IR, and 1H-NMR. Methods The inclusion compound of isoandrographolide- β-cyclodextrin was pre- pared by saturated solution method assist microwave, equilibrium constants of inclusion complexes and molar ratio of isoandrogapholide-β-cyclodextrin were determined by the UV. The inclusion compound and their molar ratio were confirmed by IR and 1H-NMR. Results The isoandrographolide and β-cyclodextrin formed inclusion compound. The equilibrium constants of inclusion complexes were 140. 845 L/mol and the molar ratio was 1 ： 1. Conclusion The isoandrographolide could be inclused by β-cyclodextrin and the inclusion effect is better.