摘要
目的研究地黄寡糖改善HepG2细胞胰岛素抵抗的分子机制。方法运用RT-PCR技术,检测地黄寡糖对胰岛素抵抗HepG2细胞内过氧化物酶体增殖物激活受体α(PPAR-α)、胰岛素受体(IR)、葡萄糖转运蛋白4(GLUT4)和2(GLUT2)基因表达的影响。结果地黄寡糖能够促进胰岛素抵抗HepG2细胞中PPAR-α、IR、GLUT4 mRNA的表达;能够明显降低GLUT2基因mRNA的表达。结论地黄寡糖对HepG2胰岛素抵抗具有明显的改善作用,其机制可能与激活PPAR-α、调节HepG2内IR及GLUT2 mRNA的表达有关。
Objective To study the molecular mechanism of Rehmannia glutinosa oligosaccharides （ROS） on the improvement of insulin resistance of HepG2 cell in vitro. Methods The mRNA expression of peroxisome proliferator-activated receptor alpha （PPAR-α）, insulin receptor （IR）, GLUT4 and GLUT2 related to insulin sensitivity was detected by reverse transcription PCR （RT-PCR）. Results The mRNA expression of PPAR-α, IR, and GLUT4 was up-regulated by ROS （10 mg/L）, but the mRNA expression of GLUT2 was inhibited. Conclusion The improving effect of ROs on the improvement of insulinresistance of HepG2 cell in vitro is related to the activation of PPAR-α and the regulation of mRNA gene expression of IR and GLUT4, as well as the inhibition of GLUT2 gene expression.
出处
《中草药》
CAS
CSCD
北大核心
2008年第8期1184-1187,共4页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(30672643,30772073)
甘肃省自然科学基金资助项目(3ZS051-A25-078)
