芦荟大黄素对T淋巴细胞增殖和细胞[Ca^(2+)]_i变化的影响

Effect of aloe-emodin on proliferation and [Ca^(2+)]_i mobilization of T lymphocytes

目的研究芦荟大黄素对植物血凝素(PHA)引起的大鼠T淋巴细胞增殖和细胞内Ca2+浓度([Ca2+]i)变化的影响,探讨芦荟大黄素对T淋巴细胞增殖的作用和机制。方法采用MTT法检测细胞增殖,用单细胞钙成像系统记录细胞胞浆[Ca2+]i。结果芦荟大黄素对PHA引起的细胞增殖有显著抑制作用,且其抑制作用与剂量有关;加入PHA引起细胞[Ca2+]i迅速升高,并持续维持高[Ca2+]i水平,芦荟大黄素对PHA引起的[Ca2+]i升高有显著抑制作用,其作用途径包括阻断胞内Ca2+释放和抑制胞外Ca2+内流。结论芦荟大黄素可抑制T淋巴细胞增殖,其作用机制可能与抑制淋巴细胞内[Ca2+]i升高相关。

Objective To investigate the effects of aloe-emodin on PHA-induced proliferation and intracellular Ca^2＋mobilization of T lymphocytes. Methods The proliferation of T cell was tested by MTT assay. The [Ca^2＋]imobilization was recorded by single cell Ca^2＋ image system using Fura-2 as Ca^2＋ probe. Results Aloe-emodin inhibited PHA-induced proliferation in a dose-dependent manner. PHA triggered a rapid [Ca^2＋]irise followed by a sustain high [Ca^2＋]ilevel. The rapid Ca^2＋ rise was caused by Ca^2＋ release from intracellular store and the sustain high[Ca^2＋]ilevel was due to Ca2＋ influx. Aloe-emodin inhibited both Ca^2＋ release and Ca^2＋ influx. Conclusion Aloe-emodin blocks PHA-induced proliferation of T lymphocytes and the mechanism for this may be due to its inhibition on PHA-induced [Ca^2＋]i mobilization.