摘要
目的研究甜菜碱对人肝癌细胞HepG2内基因组范围DNA甲基化的影响。方法以浓度0.1、0.2、0.4mol/L甜菜碱作用于人肝癌细胞HepG2 24、48 h后,通过高效毛细管电泳仪(HPCE)检测肿瘤细胞中DNA水解产物中5-甲基-2′-脱氧胞嘧啶(5-methyl-2′-deoxycytidine,5 mdC)水平,来检测甜菜碱对HepG2内基因组范围DNA甲基化表达的影响。结果随着甜菜碱的浓度增大,5 mdC占总体胞嘧啶的峰面积的百分比也相应增大,且有剂量依赖性。结论甜菜碱作为甲基供体,促进HepG2细胞基因组范围DNA甲基化的表达,从而调控细胞周期抑制肿瘤的生长。
Objective To study the effect of betaine on the DNA methylation in HepG2 genome. Methods After betaine at concentration of 0. 1, 0. 2, and 0.4 mol/mL was applied to HepG2 cells for 24 and 48 h, the level of 5-methyl-2-deoxycytidine (5 mdC) in the DNA hydrolytic product in the tumor cells was tested using HPCE. Results As the eoneentraion of betaine increased, the percentage of 5 mdC in the peak area of total cytidine also increased in a dosg-dependent manner. Conclusion As a methyl donor, betaine could promote the expression of DNA methylation in the genome of HepG2 cells, thus regulating the cell cycle of cells and inhibiting tumor growth.
出处
《中草药》
CAS
CSCD
北大核心
2008年第8期1212-1215,共4页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(30400352)
教育部科学技术研究重点项目(205045)
黑龙江省自然科学基金资助项目(D200611)
黑龙江省研究生创新基金项目(YJSCX2006-0077HSD)
关键词
甜菜碱
DNA甲基化
高效毛细管电泳仪
betaine
DNA methylation
high-preformance cpillary electrophoresis (HPCE)