转录因子Skn-1a激活人乳头瘤病毒18 E6基因表达的分析

The Transcription Factor Skn-1a Activates Human Papillomavirus Type 18(HPV-18) E6 Gene Expression

用脂质体介导真核表达质粒pcDNA3-skn-1a转染Hela细胞,实时定量PCR检测HPV18 E6基因的表达量,以探讨转录因子Skn-1a对HPV18 E6基因表达的调控.结果显示Skn-1a能够显著激活HPV18 E6基因表达,为高发宫颈癌E6基因调控的分子机理探讨提供理论依据.

To investigate the regulation of HPV-18 E6 gene expression by skn-1a , HeLa cells were transfected with skn-1a eukaryotic expression plasmid pcDNA3-skn-1a , and the transcription of HPV-18 E6 in HeLa cells was detected with real-time quantitative PCR. The results showed that skn-1a can activate the expression of HPV-18 E6 significantly.