建立大鼠常位肺肿瘤模型的新方法

New Method of Development for Rat Orthotopic Lung Tumor Model

目的应用支气管内细胞移植的方法建立大鼠肺肿瘤模型。方法40只雌性Wistar大鼠（平均体重169g±10g），用戊巴比妥钠腹腔注射麻醉大鼠，气管切开，超细导管支气管插管，接种肿瘤细胞（1×10^5～5×10^5）。病理学检查，计算肿瘤成活率。结果造模动物40只，存活39只。接种1×10^5～5×10^5个细胞各组动物的肿瘤成活率分别为60％、80％、90％和100％。结论应用支气管内细胞移植建立常位大鼠肺肿瘤模型的方法是可行的，其肿瘤成活率高，手术死亡率低，方法简单易行，易于重复。

Objective The objective of this study was to develop a rat orthotopic lung tumor model. Methods Forty female Wistar rats weighing 169 g±10 g were anesthetized intrapefitoneally with pentobarbital, tracheotomized and intubated with an uhrafine catheter for inoculation with Walker＇ s tumor cells （ 1 × 10^5 - 5 × 10^5 ）. Pathological examination was performed and the tumor take rate was determined. Results 39 rats survived among the 40 operated animals. The tumor take rates were 60%, 80%,90% and 100% for implants with 1 × 10^5 - 5 × 10^5 tumor cells respectively. Conclusion The present rat orthotopic lung tumor model was shown to be feasible： the take rate was high, surgical mortality＂ was negligible and the procedure was simple to perform and easily reproduced.