摘要
目的探索短时间内快速建立动脉粥样硬化家兔模型的方法和评价指标。方法用高胆固醇饲料喂养法与免疫反应损伤法结合建立兔动脉粥样硬化动物模型。分别于高脂饲养前、第4周和第8周测定血甘油三脂(TG)、总胆固醇(TC)、高密度脂蛋白(HDL)和低密度脂蛋白胆固醇(LDL)的含量、丙二醛的含量(MDA)、超氧化物歧化酶(SOD)、一氧化氮(NO)、C反应蛋白(CRP),并于第8周后作形态学观察。结果饲养4周后,模型组血清中甘油三脂(TG)、总胆固醇(TC)、高密度脂蛋白(HDL)和低密度脂蛋白(LDL)的含量、丙二醛(MDA)的含量、超氧化物歧化酶(SOD)、一氧化氮(NO)、C反应蛋白(CRP)(P<0.01)等显著性升高。饲养8周后模型肉眼及光镜下均可见典型动脉粥样斑块。结论此法能短时间内建立兔动脉粥样硬化模型。
Objective This article （study） was purposed to explore a method for rapid establishment of the rabbit atherosclerosis model within a short period of time and its evaluation indications. Methods The rabbit atherosclerosis model sould be prepared by the combination of feeding on hypercholesterol feed and immunological injury procedure. Before,4 and 8 weeks after the feeding on high fat diet, the following indexes should be examined： blood triglyceride （TG）, total cholesterol （TC）, high density lipoprotein cholesterol （HDL-C）and low density lipoprotein （LDL-C）, malonaldehyde （MDA）, superoxide dismutase （SOD）, nitrous oxide （NO）, C-reactive protein （ P 〈 0.01 ）. HE staining should be used to view the aterial morphological changes after 8 weeks. Results After feeding for 4 weeks, TG, TC, HDL,LDL, MDA, SOD, NO, and CRP all were increased statistically significantly （ P 〈 0.01 ）. Typical atherosclerotic plaques can be observed by both naked eyes and light microscopy. Conclusion The above-mentioned methods can be used to establish a rabbit atherosclerosis model.
出处
《实验动物科学》
2008年第3期18-21,共4页
Laboratory Animal Science
