两种抗应激剂对恒河猴抗热应激作用的试验研究

Study of The Effects of Two Anti-stressors on Rhesus Monkeys in Heat-stressed

目的研究两种抗应激剂对恒河猴热应激的影响。方法将6只恒河猴随机分为A,B两组采血做9项血液学及9项血液生化指标测定,A组日粮中每只添加VC300 mg/d和VE50 mg/d;B组日粮中添加0.2%苜草素,饲喂两个月后分别将A、B两组恒河猴放入(32±1)℃,湿度70%猴房内,1 h后采血,测定相应血液学及血液生化指标。结果两组动物血液学各项指标试验前后差异不显著(P>0.05);而血液生化值呈现:A组试验后AST、ALP、LDH分别为(101±23.64)U/L,(623.67±233.12)U/L,(1384.33±807.66)U/L,试验前后差异不显著(P>0.05);B组试验后TP、ALB分别为(83.83±16.32)g/L,(38.53±0.57)g/L;试验前后差异不显著。结论VC和VE在恒河猴抗热应激过程中主要作用于维持血液中AST、ALP、LDH的活性稳定,而苜草素主要作用于维持血液中TP、ALB的稳定,两种添加剂能有效提高恒河猴抗热应激的能力。

Objective Study of the effects of Two Anti-stressors on Rhesus Monkeys in heat-stressed . Methods Selected six health laboratory rhesus monkeys, and divided into group A, B, Nine hematological and nine blood biochemical were measured,A was added Vitamin C 300 mg/d and Vitamin E 50mg/d each in diets, B group was added mucaosu 0.2%, Two months later, Croup A and B was bred under the condition of （32 ± 1）℃,70% humidity for one hour, After that measured hematological and biochemical. Results Test cross-reference hematological was not significantly （ P 〉 0.05）, After test, AST. ALP, LDH of group A were （ 101 ± 23.64） U/L, （623.67 ±233.12 ） U/L, （1384.33 ± 807.66）U/L and was not significant than before test （P 〉 0.05）;After test, TP、 ALB of group B were （83.83 ± 16.32）g/L,（38.53 ±0.57）g/L and was not significant than before test （P 〉0.05）. Conclusion Effects of Vitamin C and Vitamin E were stood balance of AST,ALP、LDH in blood on Rhesus Monkeys in heat-stressed,Mucaosu were stood balance of TP、ALB in blood, finding two anti-stressors had effects on development anti-hotstress on rhesus monkeys.