小鼠胚胎移植研究

Study on Embryo Transfer in Mice

目的研究不同时期胚胎对胚胎移植成功率的影响以提高胚胎移植率。方法采用经超量排卵的KM雌鼠与正常615雄鼠交配,取受精卵,分别将单胚卵、双胚卵、桑椹胚和囊胚、移入发情的假孕KM雌鼠的输卵管和子宫中妊娠产仔。结果移入单胚卵、双胚卵、桑椹胚和囊胚的假孕母鼠妊娠率分别为36.36%、42.86%、10%、27.27%,产仔率分别为46.58%、38.46%、58.33%、60%。结论移植双胚卵可提高小鼠的胚胎移植成功率。

Objective The comparison of embryonic implantation in order to improve the success of embryo transfer in mice. Methods The KM mice were dealed with PMSG and HCG to make them superovulation, afterwords were mated individually with the male mice. The fertilized eggs were collected from the plugged females. Embryos from the one-cell stage,two-cell stage, morula and blastula were transferred to pseudopregnant recipients. Results By transferring embryos from one-cell stage, two-cell stage, morula and blastula, the rates of pregnancy were 36.36 % ,42.86 %, 10 % ,27.27 %, the birth rates were 46.58 %, 38.46 % ,58.33 % ,60 % . Conclusion More embryos from the two-cell stage develop to term than those from the other stage