银屑病患者外周血树突状细胞功能的体外研究

Study in vitro on specific immune function of dendritic cells in peripheral blood of patients with psoriasis vulgaris

目的研究寻常型银屑病患者外周血树突状细胞(DC)激活及促进T淋巴细胞分化的功能。方法用体外培养细胞法培养DC,流式细胞术检测DC细胞表面标志,ELISA法检测培养细胞上清中IL-12、IL-4、IFN-γ和IL-1β的含量。结果银屑病患者DC与正常人相比,其细胞表面标志CD83和CD86表达率明显升高(P<0.001),分泌IL-12明显增多(P<0.05),IL-4和IL-1β分泌明显减少(P<0.001),IFN-γ分泌无差异。结论寻常型银屑病患者外周血DC的抗原提呈能力和激活T淋巴细胞的功能增强,而且促进TH0向TH1分化的能力增强。

Objective To investigate the capability of dendritic cells （DCs） from peripheral blood of the patients with psoriasis vulgaris for stimulating T lymphocytes to proliferate and differentiate in vitro. Methods DCs of peripheral blood in patients with psoriasis vulgaris were cultured for five days with medium containing GM-CSF and IL-4. CD83 and CD86, the surface markers of DCs, were detected by flow cytometry. The levels of IL-12, IL-4, IL-1β and IFN-γ in the cell culture supernatant were detected by ELISA. Results Com- pared with health group, the expression of CD83 and CD86 on DCs in the patient group were all increased evidently （P 〈0.01 ）. The level of IL-12 was increased markedly （P 〈 0.05 ）, but the productions of IL-4 and IL-1 β were decreased obviously （P 〈 0. 001 ）. No difference was found for the production of IFN-γ. Conclusion The capability of antigen presentation and of DCs in the patients with psoriasis vulgaris was increased evidently, which promoted the activation and differentiation of T lymphocytes from Th0 to Th1.