EMMPRIN、MMP-2、TIMP-2在子宫内膜异位症中的表达及意义

Expression and Significance of EMMPRIN,MMP-2 and TIMP-2 in Endometriosis

目的探讨细胞外基质金属蛋白酶诱导因子(EMMPRIN)、明胶酶-A(MMP-2)和基质金属蛋白酶组织抑制剂(TIMP-2)在子宫内膜异位症(EMs)的表达和意义。方法应用免疫组化二步法检测36例EMs患者的异位内膜、在位内膜及20例非EMs患者的对照正常内膜中的EMMPRIN、MMP-2、TIMP-2的表达情况,并对它们的EMM-PRIN、MMP-2、TIMP-2蛋白表达水平进行相关性分析。结果异位内膜组EMMPRIN、MMP-2阳性表达率明显高于在位内膜组和对照正常内膜组(P<0.05);异位内膜组TIMP-2阳性表达率明显低于在位内膜组和对照正常内膜组(P<0.05)。在异位内膜组中,EMMPRIN和MMP-2呈正相关性(P<0.01);MMP-2和TIMP-2呈负相关,但一致性较差(P>0.05)。结论EMMPRIN、MMP-2和TIMP-2共同参与了子宫内膜异位症的发生;MMP-2在异位内膜的高表达使异位内膜具有更高的侵袭性,在子宫内膜异位症的病程发展进程中起着重要作用。

Objective To explore the expression and significance of EMMPRIN, MMP - 2 and TIMP - 2 in endometriosis. Methods Immunohistochemical method was employed to detect the expression of EMMPRIN, MMP - 2 and TIMP - 2 in the ectopic endometrium of 36 patients with endometriosis, the eutopic endometrium of 36 patients with endometriosis and the normal controls （n= 20）, then the correlation of the expression of them was analyzed. ResuIts The positive expression rates of EMMPRIN and MMP - 2 in the ectopic endometrium group were significantly higher than those of the eutopic endometrium group and the normal endometrium group （ P〈 0.05）. The positive expression rate of TIMP - 2 in the ectopic endometrium group was significantly lower than that of the eutopic endometrium group and the normal endometrium group （P 〈 0.05）. There was a positive correlation between the expression levels of EMMPRIN and MMP - 2 in the ectopic endometrium group （ P〈 0. 01 ）, and there was a negative correlation between the expression levels of MMP - 2 and TIMP - 2, but the consistency was poor （P〈 0.05）. ConeIusions EMMPRIN, MMP- 2 and TIMP 2 are all involved in the process of invasion and tissue remodeling, which is supposed to be part of pathogenesis of endometriosis. The high expression of MMP - 2 in the ectopic endometrium suggests that the ectopic endometrium with endometriosis may be more invasive and prone to peritoneal implantation.