幽门螺杆菌相关性胃炎与胃粘膜TNF-α关系的探讨

Relationship Between Antral Mucosal Tumor Necrosis Factor-α concentration and Helicobacter Pylori-associated Gastritis

目的探讨胃粘膜肿瘤坏死因子-α(TNF-α)含量与幽门螺杆菌(Hp)相关性胃炎的关系。方法23例Hp(+)胃炎患者,22例Hp(-)胃炎患者,20例胃粘膜正常者,均经胃镜和病理组织学检查确诊。23例Hp(+)胃炎患者均接受1周抗Hp治疗。利用ELISA法检测胃粘膜培养上清液中TNF-α的水平。结果Hp(+)胃炎患者胃粘膜组织上清液中的TNF-α含量明显高于Hp(-)胃炎患者组和对照组,差异均具有显著性(P<0.05)。活动性胃炎患者胃粘膜组织Hp感染率和上清液中的TNF-α含量均明显高于非活动性胃炎患者,差异具有显著性(P<0.05)。治疗后17例Hp根除患者中,TNF-α含量明显低于治疗前,差异具有显著性(P<0.05)。结论Hp感染可明显诱导胃粘膜TNF-α的表达;TNF-α在活动性胃炎发生中起到重要作用。

Objective To investigate the relationship between antral mucosal tumor necrosis factor- α（TNF - α） and Helicobacter pylori（Hp）associated gastritis. Methods Twenty - three patients with Hp associated gastritis, 22 patients with Hp negative gastritis, and 20 normal controls were confirmed by endoscopic and pathologic examination. Twenty-three patients with Hp associated gastritis accepted treatment against Hp infection for a week. Gaster antral mucosal cultured supernatant was collected to detect TNF - α using ELISA. Results The level of TNF - α in supernatant was higher in patients with Hp associated gastritis than that in patients with Hp negative gastritis and normal controls（P〈 0.05）. The infection rate of Hp and level of TNF - α in supernatant were higher in patients with active gastritis than those in patients with inactive gastritis （P〈 0.05）. The level of TNF-α in supernatant was decreased from 17 patients with Hp associated gastritis after the treatment against Hp infection（P〈 0.05）. Conclusions TNF - α is highly expressed in antral mucosa after Hp infection, which may play an important role in the pathogenesis of chronic gastritis.