水引发对紫花苜蓿种子萌发及其生理活动的影响

Effect of hydro-priming on seed germination and physiological activities in Medicago sativa

采用4个紫花苜蓿品种三得利、阿尔冈金、新疆大叶和陇东种子,研究了控制加水引发对紫花苜蓿种子萌发及几个生理指标的影响。结果表明,引发显著缩短了所有供试紫花苜蓿品种种子达最终发芽率50%的萌发时间(P<0.01),提高种子发芽指数,但对最终的发芽率和硬实率无显著影响。引发显著降低了所有参试品种种子可溶性糖含量、种子水浸8 h时电导率(P<0.05),增加了所有参试品种种子脯氨酸含量;引发种子丙二醛含量虽然略低于未引发种子,但差异不显著。这说明紫花苜蓿种子水引发不仅能够加快种子萌发速率,提高萌发一致性,而且能够修复生物膜、增强细胞结构稳定性并增强种子抗逆性。

The effects of hydro-priming on seed germination and physiological activities in four Medicago sativa varieties （Sandili, Algonquin, Xinjiangdaye, Longdong） were studied. Hydro-priming significantly shortened the time for 50% seed germination and promoted the germination index of seeds but had no significant effect on germination percentage and hard seed content. Hydro-priming reduced the soluble sugar content and electrolyte leakage, and increased the proline content of seeds. The malondialdehyde content in primed seeds was lower than that of non-primed seeds, but not significantly so. It is concluded that hydro-primed M. sativa seed germinated faster and more uniformly than non-primed seeds. Hydro-priming repaired the biological membrane, enhanced the stability of cellular structure and resistance to germination stress.