阿托伐他汀对自发性高血压大鼠血压的影响

Effects of atrovastatin on blood pressure in spontaneously hypertensive rats

目的:观察阿托伐他汀对自发性高血压大鼠(SHR)血压的影响,初步探讨其调节血压的机制。方法:SHR(n=12)随机分为SHR对照组、阿托伐他汀治疗组,同龄WKY大鼠(n=6)作为正常血压对照组,灌胃给药6周。采用尾袖法测量大鼠尾动脉收缩压,硝酸还原酶法测定血清NO浓度,化学比色法检测NOS活性和ROS活性,放免分析法测定心肌AngⅡ含量,透射电镜观察主动脉内皮超微结构改变。结果:阿托伐他汀治疗6周后,SHR治疗组SBP明显下降(P<0.01);心肌AngⅡ浓度明显下降(P<0.05);血清NO浓度和主动脉NOS活性明显增高(P<0.01,P<0.05),血清ROS水平明显降低(P<0.05)并明显改善SHR主动脉内皮的超微结构改变。结论:阿托伐他汀可降低SHR血压,可能是通过增加主动脉NOS活性,升高血清NO含量,减少ROS生成,改善血管内皮功能而发挥降低血压作用。

Objective：To investigate the effects of atorvastatin on blood pressure in spontaneously hypertensive rats （SHR） for exploring the potential mechanisms. Methods： SHR （ n = 12） were randomly divided into SHR control and atorvastatin group, Wistar-Kyoto rats of agematched were selected as normal control group with measurement of the systolic blood pressure by tail-cuff method. Nitric oxide （NO） and reactive oxygen species （ROS） levels in serum, Ang Ⅱ levels in myocardium and nitric oxide synthase （NOS） activity in aortic were measured by ni- trate reductase method, chemistry colorimetry, raddioimmunossay respectively. Transmission electron microscope was used to observe the aortic ultrastructure. Results ： After 6 weeks treatment,as compared atorvastatin grou with SHR control group, SBP, the AngⅡ levels in myocardium and serum and ROS levels was significantly decreased （ P 〈 0.01, P 〈 0.05）. The levels of NO and NOS were remarkably significantl increased in atorvastatin group（ P 〈 0.01, P 〈 0.05）. It was ob- served in atorvastatin group that ultrastmctural in aortic endothelial cell were improved. Conclusion ： That atorvastatin can cause a significant re- duction in blood pressure in SLIP, is that it may increase the aortic NOS activity and serum NO concentration by decreasing ROS activity and improving endothelial dysfunction.