救必应抑菌抗炎有效部位筛选

Screening the Effective Part of Holly Bark for Bacteriostasis and Anti-inflammatory Effect

目的:提取分离救必应,得到各不同的极性部位,进行抑菌和抗炎实验筛选。方法:以不同极性的有机溶剂提取分离救必应,各部位用试管稀释法抑菌筛选,效果显著部位先进行二甲苯诱导小鼠耳肿胀和棉球诱导大鼠肉芽肿的抗炎实验筛选,再以硅胶柱层析洗脱分离,进一步抑菌实验筛选。结果:救必应乙醇提取部位具有较强的抑菌和抗炎作用,蒸馏水提取部位有较强抑菌作用,但无抗炎作用。柱层析分离筛选之后,抑菌部位主要发生在乙醇提取部位以甲醇:氯仿(1:1)洗脱部分,水提取部位90%乙醇和70%乙醇洗脱部分。

Objective：To extract and separate each different polarity part of Holly Bark, then screen the effective part for baeteriostasis and anti- inflammatory effect. Methods ： Use different polarity organic solvent to extract and separate Holly Bark. The diffent part has been run tube dilution method experiment for screening baeteriostasis part. At first the effective part has been run to screen anti - inflammatory effect experiment which eontains Xylene - induced mouse ear swelling test and Cotton-induced rat granuloma test. Then Silica gel column chromatography elut Separation, and antibacterial farther experimental screen. Results： It should save the ethanol extract had strong antibaeterial and anti-inflammatory effects,distilled water extract had a strong inhibitory effect, but no anti-inflammatory effect. Column chromatography screening,the main inhibitory site in ethanol extracts of methanol：chloroform （1：1 ） elution,water extracts 90% ethanol and 70% ethanol elution part.