芸薹属种间F_1与羽衣甘蓝回交亲和性及子房培养研究

Studies on Backcross Compatibility Between Brassica Hybrid F_1 and B. oleracea var. acephala and Ovary Culture

以大白菜与羽衣甘蓝CMS种间杂种F1为母本,以羽衣甘蓝自交系为父本进行回交,并对杂交子房进行了离体培养。结果表明:上述亲本间存在较强的生殖隔离,花期杂交不结实,蕾期重复授粉提高了亲和力。授粉后9 d的杂交子房进行挽救培养效果最佳,离体条件下结籽率10%。其次为授粉后12 d及6 d的子房,结籽率分别为5%和4.4%。外源激素及其配比对子房离体结实具有极显著影响,以BA 2+NAA 0.1最佳,结籽率为9%;其次为BA 2+NAA 0.2,结籽率为3%;BA 2+NAA 0.3及无激素MS培养基上均未获得饱满种子。

The paper studied on backcross between Brassica hybrid F1 and B. oleracea var. acephala. The results indicated that there was an obvious segregation between the above parents, which could not give birth to new seeds normally. More times pollination at bud stage enhanced compatibility. The ovaries from that of 9 days after pollination was the best time of materials, with the rate of seeds 10%. Ovaries of 12 days and 6 days after pollination were secondary, with the rates of seeds 5% and 4.4% respectively. Hormone prescriptions had marked effects to achieving seeds, where BA 2＋ NAA 0. 1 did best, with the rate of seeds 9%, then BA 2＋NAA 0.2. BA 2＋NAA 0.3 and empty MS did not succeed.