榅桲的组织培养初报

Primary Study on Tissue Culture of Cydonia oblonga Mill

以苹果型榅椁嫩枝茎段为试材，研究了不同灭菌剂、灭菌时间、基本培养基、激素组合等对榅桲外植体灭菌、初代培养、继代培养和生根培养的影响。结果表明：用0．1％升汞溶液消毒5min，灭菌效果最好；初代培养基以MS＋6-BA0．6mg／L效果较好,萌芽率和增殖倍数均较高，分别为93．3％和4．7倍；继代培养基以MS＋6-BA 0．6mg／L＋IBA 0．2mg／L和MS＋6-BA 0．6mg／L＋IBA 0．4mg／L组合交替使用更有利于组培苗的增殖和壮苗；生根培养基以1／2 MS（I）＋NAA1．0mg／L生根率最高达50％，平均根数为7～10根；短时间（7d）暗培养对诱导韫椁苗生根有利，随着暗培养天数的增加叶焦边现象逐渐加重甚至出现顶端枯死现象；6-BA对诱导韫柠组培苗生根无益且易出现畸形叶。

Using the tender-stem of Cydonia oblonga Mill to have a study on the effect of different disinfectant, sterilization time,culture medium, hormone combinations, etc. The results showed that （1）Using HgCl2 0.1% for 5 min was the best for sterilization; （2）The better Primary medium was MS＋6-BA 0. 6 mg/L and its bud ratio and multiplication mul- tiple were all higher ,they were 93. 3% and 4. 7; （3）Using MS＋6-BA 0. 6 mg/L ＋IBA 0. 2 mg/L and MS＋6-BA 0. 6 mg/L＋IBA 0. 4 mg/L by turns was better for multiplication and strong seedlings; （4）Rooting medium chose 1/2 MS （I）＋NAA1.0 mg/L can achieve the highest 50% rootage rate and 7-10 average root number; （5）Short dark treatment（7 d） was benefit for rooting but as the time longer the leaf--burnt became more serious and even lead to dead of stem tip; （6）6--BA was not benefit for rooting and even lead to abnormal leaves.