真菌诱导子对春兰组培物生长的影响

Effects of Fungal Elicitors on the Growth of the Tissue Culture of Cymbidium Goeringii

在1/2 MS基本培养基中,分别加入不同种类、剂量的由野生春兰根部分离到的内生真菌所制成的干菌丝诱导子,对由春兰种子萌发成的原球茎、根状茎等组培物进行诱导培养。结果表明,4种真菌诱导子均可不同程度的促进春兰组培物生长量的增加及不定芽的分化,其诱导作用依次为:CF11>CF3>CF1>CF8>CK。加入CF11、CF3号诱导子的处理与对照相比,鲜重分别增加了74.38%、73.93%,诱导效果最好。此外,春兰组培物的鲜重随着CF1号诱导子加入量的增大而增加,其诱导作用依次为20 g/L>10 g/L>5 g/L>0 g/L,加入量为20 g/L时,其鲜重与对照相比达到了显著水平。

Four different fungus strains that were isolated from the roots of Cymbidium goeringii are made of dry mycelial elicitor. Tissue cultures of Cymbidium goeringii were induced on basal culture medium of 1/2 MS with the fungal elicitor. The result showed that different fungual elicitor promoted the growth and differentiation of adventitious bud of tissue culture in different degree, the inducing effects were in the order of CF11〉CF3〉CF1〉CF8〉CK. CF11 and CF3 did the best, and the fresh weight have increased by 74.38%and 73. 93%compared to the control. In addition, the fresh weight of tissue culture increased with adding fungal elicitor of CF1, the inducing effects were in the order of 20 g/L〉 10 g/L〉5 g/L〉0 g/L. The fresh weight had significant difference compared with those in control when the fungal elici tor added to 20g/L.