地生兰组培快繁技术研究

Study on the Quick Multiplication of Terrestrial Orchid

利用地生兰蒴果生产种子苗及切取优良母株的侧芽进行茎尖诱导繁殖,筛选出适合云南几种地生兰种子生长和茎尖培育及分生苗生长的培养基配方和组培快繁技术。适合种子发芽培养基为1/2 MS+NAA 0.3 mg/L+IBA 3.0 mg/L+活性炭(AC)2 g/L+1 g/L兰花培养液(泰国购入);茎尖培养基配方为1/2 MS+NAA 0.4 mg/L+6-BA 3.0 mg/L+活性炭(AC)2 g/L+新鲜椰子汁(CM)250 mL/L+1%葡萄糖+微量维生素C,平均诱导原球茎个数3.9/个;丛生芽的诱导1/2 MS+NAA 0.4 mg/L+6-BA 4.0 mg/L+AC 2 g/L;丛生芽生根1/2 MS+NAA 0.2 mg/L+AC 2 g/L。

The seeds and the top of stalk of terrestrial orchid as explants were cultured on medium. The tissue culture of terrestrial orchid were studied. The results were as follows. The best culture medium for seed germinate of terrestrial orchid was 1/2 MS＋NAA 0.3 mg/L＋IBA 3.0 mg/L ＋active carbon（AC） 2 g/L＋1 g/L（culture liquid of the orcbifd from Thailand）;The best medium in inducing protocorm was 1/2 MS＋NAA 0. 4 mg/L＋6-BA 3. 0 mg/L ＋（AC） 2 g/L＋fresh coconut juice （CM） 250 mL/L＋1% glucoseS-micro-vitamin C,The best medium in adventitious buds induced was 1/2 MS＋NAA 0. 4 mg/L＋6-BA 4.0 mg/L ＋AC 2 g/L;The best medium in rooting of adventitious buds was 1/2 MS＋NAA 0. 2 mg/L＋AC 2 g/L.