摘要
以不同浓度(0.01、0.03、0.05 mol/L)和不同处理时间(1、3、5 h)的化学诱变剂甲基磺酸乙酯对蚕豆根尖进行处理,诱导蚕豆发生染色体结构畸变,观察、统计其根尖染色体的畸变情况,筛选出最佳诱导浓度和时间,为本科遗传教学提供理论依据。结果表明:0.03 mol/L的甲基磺酸乙酯处理1 h,诱导蚕豆根尖细胞染色体变异效果明显,可作为最佳诱导浓度和时间。
The root tip of Vicia faba L. was treated by ethylmethane sulfonate with different consistency (0. 01,0. 03, 0.05 mol/L) and different time (1,3 ,5 h). Chromosomal aberration of Vicia faba L. was induced and statistic, in order to screen out the optimum consistency and time. The results showed: there was an obvious effect on chromosome variation of Vicia faba L. treated by ethylmethane sulfonate of 0. 03 mol/L and 1 h, which could be the optimum consistency and time.
出处
《北方园艺》
CAS
北大核心
2008年第8期184-185,共2页
Northern Horticulture
基金
黑龙江省高等教育科学研究"十一五"规划课题资助项目(115C-369)
关键词
蚕豆
甲基磺酸酯(EMS)
染色体畸变
Vicia faba L.
Ethylmethane sulfonate(EIMS)
Chromosomal aberration