摘要
以不同浓度(0.01、0.03、0.05 mol/L)和不同处理时间(1、3、5 h)的化学诱变剂甲基磺酸乙酯对蚕豆根尖进行处理,诱导蚕豆发生染色体结构畸变,观察、统计其根尖染色体的畸变情况,筛选出最佳诱导浓度和时间,为本科遗传教学提供理论依据。结果表明:0.03 mol/L的甲基磺酸乙酯处理1 h,诱导蚕豆根尖细胞染色体变异效果明显,可作为最佳诱导浓度和时间。
The root tip of Vicia faba L. was treated by ethylmethane sulfonate with different consistency （0. 01,0. 03, 0.05 mol/L） and different time （1,3 ,5 h）. Chromosomal aberration of Vicia faba L. was induced and statistic, in order to screen out the optimum consistency and time. The results showed： there was an obvious effect on chromosome variation of Vicia faba L. treated by ethylmethane sulfonate of 0. 03 mol/L and 1 h, which could be the optimum consistency and time.
出处
《北方园艺》
CAS
北大核心
2008年第8期184-185,共2页
Northern Horticulture
基金
黑龙江省高等教育科学研究"十一五"规划课题资助项目(115C-369)
关键词
蚕豆
甲基磺酸酯(EMS)
染色体畸变
Vicia faba L.
Ethylmethane sulfonate（EIMS）
Chromosomal aberration
