贴梗海棠组培快繁技术研究

Studies on Tissue Culture and Rapid Propagation Technique of Chaenomeles speciosa(sweet)Nakai

以贴梗海棠带芽茎段为材料,研究了贴梗海棠组织培养和快速繁殖的适宜条件,结果表明:茎尖和半木质化茎段在MS+6-BA 1 mg/L+NAA 0.2 mg/L中的诱导率均在90%以上;筛选出的最佳继代增殖培养基为:MS+6-BA 0.5 mg/L+NAA 0.5 mg/L,增殖系数为7.2;生根培养基为:1/2 MS+IBA 0.3 mg/L+NAA 0.2 mg/L,生根率达93%。

By using stems with buds of Chaenomeles speciosa （sweet）Nakai as materials ,the experiment was made to find out the best culture process and proper culture medium. The technical system of tissue culture for rapid propagation was established. The results were as follows：The rate of initiation of shoot apex and half-ligtfification stems in MS＋6-BA 1 mg/L＋NAA 0. 2 mg/L were all over 90%; The optimal subculture medium was MS＋6-BA 0. 5 mg/L＋NAA 0. 5 mg/L, the multiplication rate of shoot clumps could be up to 7. 2; The proper rooting medium was 1/2 MS＋IBA 0.3 mg/L＋NAA 0. 2 mg/L, and the rooting rate could be up to 93%.