几种苏云金芽孢杆菌杀虫晶体蛋白对黑龙江省主要蔬菜害虫小菜蛾活性分析

Analysis Activity of Cry Protein from Bacillus thuringinesis Against Plutella xylostella of Vegetable Pest in Heilongjiang Province

利用已克隆的3种Bt cry基因cry1Ca、cry1Ia、cry2Ab和1种野生菌株HD-73(cry1Ac)表达的4种Bt Cry杀虫晶体蛋白,对小菜蛾进行生物活性分析,并将Cry1Ac杀虫晶体蛋白分别与其它3种蛋白按1∶1的比例组合,对小菜蛾进行生物活性测定。结果表明:单独使用Cry1Ac的活性最高,LC50为2.39μg/mL,Cry1Ac与Cry2Ab混合对小菜蛾的具有较高的毒力,LC50为48.91,高于其它组合。

Using expression protein of cry2Ab, crylIa, crylCb which had been cloned and a nature strain HD- 73 （cry1Ac） against Plutella xylostella. And mixed CrylAc with one of the other three Cry proteins（1 = 1） against Plutella xylostella. The bioassay result demonstrated that CrylAc were higher than other three, and LCA0 was 2.39ug/mL. The LC50 of co-toxicity of Cry1Ac and 2Ab to Plutella xylostella was 48. 91ug/mL. It was meant that Cry 1Ac and Cry2Ab were high toxicity than the other Cry proteins combinations.