黑龙江0288松茸菌株生物学特性

Biological Characteristic of Tricholoma motsutoke Heilongjiang 0288

以紫外线诱变松茸孢子获得的菌株0288其生长速度和生物积累量为指标,选择优良适于液态发酵松茸菌株为目的,生长速度快平均菌丝生长量为0.575 mm/d;在0.51、和2 h下提取到的多糖含量分别为3.56%、8.14%和8.46%;100 g菌丝的干重为10.56 g。液体培养基以G培养基提供的氮源较适合松茸生长:在0.51、和2 h下提取到的多糖含量分别为3.9%、8.59%和8.73%;100 g菌丝的干重为7.34 g。液体培养基比母种培养基更适合0288松茸菌丝的生长,发酵罐的批量培养是对液体培养的优化,与其它供试株比较差异极显著,经人工培养获得了松茸子实体。

Took growth speed and biomass of Tricholoma rnotsutoke Heilongjiang 0288 as the selection standard, and the selection of Tricholoma rnotsutoke strain that can be used in liquid fermentation as a goal,one strain obtained from spore separation and then ultra-violet mutation showed high growth speed with average mycelia growth speed of 0. 575 mm/d. The contents of polyscharides in the samples were 3.56 %, 8. 14%,8. 46% respectively when the distilled time was 0. 5, 1,2 h. Dry weight per 100 g wet mycelium was 10. 56 g. Media Gproviding suitable nitrogen source for the mycelium growth was the best media in this story. The selected Trickoloma rnotsutoke strain formed mushroom balls on the fourth day and its mushroom balls were the biggest on the fifth day. The contents of polysaccharides in the samples were 3. 9 %, 8.59 %,8. 73 % respectively when the distilled time was 0. 5,1,2 h. The mycelia dry weight reached 8. 14 %/100g. Liquid media were better than solid stock media for the growth Tricholorna rnotsutoke strain 0288, and the optimal condition for liquid mycelium culture was batch culture with fermentation tin. This strain had significant difference in mycelium dry weight and conetents of polysaecharides from other stains, and Tricholoma rnotsutoke fruit-body were cultured.