热性惊厥大鼠体内脑源性神经营养因子及核因子-κB的变化

BDNF/NF-κB signal pathway activation in rats with febrile seizures

目的研究反复热性惊厥（FS）后脑源性神经营养因子／核因子-κB（BDNF／NF-κB）信号通路的改变。方法51只雄性SD大鼠随机分为正常对照组（NC组，n=14）、高热对照组（HC组，n=19）和热性惊厥组（FS组。n=18）。水浴法建立FS模型。酶联免疫吸附法与免疫组织化学法检测血清与海马BDNF、NF-κB的表达。结果酶联免疫吸附法检测结果显示。FS组血清与海马BDNF水平均明显高于NC组和HC组（P值均〈0．01）。免疫组织化学法检测结果显示，FS组各脑区BDNF阳性神经元明显增多，且其光密度值明显高于NC组和HC组（P〈0．01）；FS组各脑区NF-κB阳性细胞光密度值亦均明显高于NC组和HC组（P〈0．01）；HC组胞核中NF-κB表达和光密度值也均明显高于NC组（P〈0．01）；脑组织各区BDNF的表达与NF-κB的表达呈正相关关系（r=0．78，P〈0．01）。结论反复FS后BDNF、NF-κB表达均明显增加，且二者呈正相关，提示FS发病过程中BDNF／NF-κB信号通路可能被激活。

Objective To study the change of BDNF/NF-κB signal pathway induced by repeated febrile seizures. Methods Fifty-one 21 d male rats were randomly divided into three groups： normal control group （n = 14）, hyperthermal control group （n = 19） and febrile seizure group （n = 18）. The febrile seizure model was developed by warm water immersion [ （45.0 ± 0.3） ℃ ]. BDNF and NF-κB were measured by enzyme linked immunosorbent assay （ELISA） and immunohistochemistry. Results The levels of BDNF in serum and hippocampus in FS group were higher than those in NC group （P〈0.01） and in HC group （P〈 0.01）. In FS group, the OD of the BDNF positive neuron was higher than that of NC group （P〈0.01） and HC group （P〈0.01） ;the OD of the NF-κB positive neuron in FS group was higher than that of NC group （P〈 0.01 ） and HC group （P 〈 0.01 ）, the OD of the NF-κB positive neuron in HC group higher more than in NC group （ P 〈 0.01 ）. There was positive correlation between BDNF expression and NF-κB activation （ r = 0.78, P 〈 0.01 ）. Conclusion The expression of BDNF and NF-κB were obviously increased in FS group and they are positively correlated, suggesting that the BDNF/NF-κB signal pathway may be activated after febrile seizure.