虾类和蟹类过敏原的交叉反应性研究

Study on cross-reactivity of allergens between shrimps and crabs

目的:分析虾类和蟹类过敏原交叉反应性,探讨其在食物过敏症的预防、诊断和治疗中的意义。方法:采用SDS-PAGE分析虾蟹的蛋白组分,Western blot分析其过敏原组分,Western blot和ELISA抑制试验研究虾类和蟹类的过敏原交叉反应性。结果:虾类、蟹类过敏原蛋白组分相似性与其种属关系相关,相对分子量(Mr)在20 000、36 000、38 000、44 000、68 000、75 000、85 000处具有相同条带;Mr为36 000的蛋白是虾的主要过敏原,Mr为36 000、66 000、85 000的蛋白是蟹的主要过敏原;青蟹蛋白和日本沼虾蛋白对其余虾蟹过敏原有抑制作用,并且随着抑制物浓度增加抑制效应增强。结论:虾类和蟹类过敏原有相似性,其中Mr在36 000处相互之间存在较强的交叉反应。

Objective： To explore the mechanism for the immune cross-reaction between crabs and shrimps. Methods： The cross-reactivity between shrimps and crabs was observed by inhibition of ELISA and Western blot. Results： The protein constituents were similar where their genus relationship was closer in shrimps and crabs. There were the same bands at 20,36,38,44,68,75, and 85 kD of molecule weight. The protein at 36 kD was the main allergen for shrimps and the proteins at 36,66 and 85 kD were the main allergens for crabs. S. serrata and M. nipponense could inhibit the allergic response of various kinds of shrimp and crab in a concentration-dependent pattern. Conclusion： There is a strong immune cross-reaction between different species of shrimp and crab in 36 000.