高分辨阿达玛变换显微光谱成像系统研究

Study on the High-resolution Hadamard Transform Microscopic Spectral Image System

阿达玛变换(HT)是一种多通道的光谱调制技术,基于这一原理,研制了一种显微光谱成像系统。通过光学设计,除可以获得微小试样(如单个细胞)的光谱以外,还可以对试样进行分光谱成像,得到分辨率为256×256像素的256级灰度图像。通过对两种不同荧光发射波长的CdSe/ZnS量子点进行的分光谱成像测试表明,本系统具有良好的分光谱成像能力,可以对具有复杂光谱的物质针对其某一特定成分进行成像。

A microscopic spectral image system was developed based on the Hadamard transform （HT） multiplexing spectrum modulation technique. Not only high-resolution spectrum but also 256 × 256-pixel 256-gray-level microscopic spectral images of the micro-samples such as single cells, could be obtained with the system. In this study, the images captured at a series of wavelength of two kinds of CdSe/ZnS quantum dots with different fluorescence emission wavelength indicate that the system has excellent spectral imaging ability and can be used to image a specific component inside a sample with complicated spectrum.