高效液相色谱-蒸发光散射检测法分析何首乌中磷脂类化合物

Determination of Phospholipids in Polygonum Multiflorum Thumb by High Performance Liquid Chromatography-Evaporative Light Scattering Detection

建立了高效液相色谱测定何首乌中磷脂类化合物的新方法。采用Nuclesil100—5色谱柱（250×4．6mmi．d．，5μm），以甲醇为流动相，流速0．8mL／min，等度洗脱。蒸发光散射检测器的气体流速1．8L／min，漂移管温度66℃，进样量15ML。四种磷脂类化合物的线性范围分别为：磷脂酰甘油（PG）：0．0388～0．388mg／mL（r^2=0．9991），磷脂酰乙醇胺（PE）：0．0500～0．250mg／mL（r^2=0．9996），磷脂酰胆碱（PC）：0．0103～0．165mg／mL（r^2=0．9995），溶血磷脂酰胆碱（LPC）：0．0103～0．343mg／mL（r^2=0．9995）。该方法应用于首乌中磷脂类化合物的分析，取得了较好的结果。

A high performance liquid chromatographic method was developed to determine the phospholipids in Polygonurn rnultiflorurn Thumb. Separation was performed on a Nuclesil 100-5 column （250 × 4. 6 mm i. d. , 5 μm） , using methanol as mobile phase with a flow rate of 0. 8 mL/min. The carrier gas flow rate and drift tube temperature of evaporative light scattering detection were set at 1.8 L/min and 66℃, separately. The injecting volume was 15 μL. The linear ranges for different phospholipids were 0. 0388-0. 388 mg/mL （r^2 =0. 9991） for phosphatidylglycerol（PG）, 0. 0500-0. 250 mg/mL （r^2 =0. 9996） for phosphatidylethanolamine（PE）, 0. 0103- 0. 165 mg/mL （r^2 = 0. 9995） for phosphatidylcholine （PC）, 0. 0103 - 0. 343 mg/mL （re = 0. 9995） for lysophosphatidylcholine （LPC）. The proposed method was successfully applied to the determination of phospholipids in Polygonurn multi florum Thumb samples.