析相微萃取-石墨炉原子吸收法测定生物样品中痕量镉

Determination of Trace Cadmium in Biological Samples with Microsphere Phase Separation Extraction by GFAAS

本文以双硫腙为络合剂，乙酸乙酯为萃取剂，建立了析相微萃取-石墨炉原子吸收光谱法测定环境生物样品中痕量镉的分析方法。考察了析相规律及影响萃取的酸度条件及络合剂用量等因素，方法线性范围为0．005～0．20μg／L，相对标准偏差（RSD）为4．0％（n=7），与未经析相微萃取方法测定相比，灵敏度提高了20倍。所建立的方法用于测定国家标准物质杨树叶、灌木枝叶、人发中镉的含量，结果与参考值具有良好的一致性。

A method for the determination of Cd graphite furnace atomic absorption spectrometry with microsphere phase （GFAAS） in biological separation extraction by samples was developed. Dithizone was used as complexant and ethyl acetate as extractant. Under the optimized conditions, the linear range of the method was from 0. 005μg/L to 0.20μg/L, and the detection limit was 0. 001 μg/L, with the relative standard deviation （RSD） of 4.0% （n=7）. The signal obtained with the microsphere phase separation extraction was improved 20 times comparing with normal GFAAS. The standard biological samples were determined by this method with a good accordance to the reference values.