血红蛋白-维生素B6-ONOO-体系的荧光特性及ONOO-分析

Fluorescence Characteristic of Hemoglobin-Pyridoxine-Peroxynitrite and Its Application in the Determination of Peroxynitrite

在中性pH值的缓冲溶液中，血红蛋白作催化剂，维生素B。与过氧亚硝酸活性氧（ONOO-）发生反应，维生素B。原来位于391nm处的强烈的荧光发射峰消失，而在384nm和424nm处出现了两个新的荧光峰。据此建立了血红蛋白～维生素B。体系高灵敏检测ONOO-活性氧的新方法。方法的线性范围为5．24×10^-8～2．62×10^-6mol／L，检出限为2．62×10^-9mol／L，对1．31×10^-7mol／L的ONOO^=溶液测定的相对标准偏差为3．759／6（n=7）。

In neutral pH medium, the fluorescence characteristic of a novel fluorimetry system hemoglobin-pyridoxine-peroxynitrite（ONOO-） has been studied. With the reaction between pyridoxine and peroxynitrite, two new fluorescence emission peaks apear at 384 nm and 424 nm respectively, while the fluorescence emission peak of pyridoxine at 391 nm disappears. The fluorescent product was monitored by fluorimetry. A linear calibration graph was obtained over an ONOO- concentration range from 5. 24×10^-88 mol/L to 2. 62 5×10^-6mol/L, with a detection limit of 2. 62 ×10^-9mol/L. The relative standard deviation at an ONOO- concentration of 1. 31×10^-7 mol/L was 3.75% （n=7）.