高效液相色谱法测定感冒止咳颗粒中黄芩苷的含量

Determination of Baicalin in the Granules of Ganmao Zhike by HPLC

采用高效液相色谱（HPLC）法，用C18色谱柱，甲醇-水-冰乙酸为（55：45：1，V／V／V）流动相，在波长276nm处测定感冒止咳颗粒中黄芩苷的含量。黄芩苷在0．101～1．01μg范围内与峰面积有良好的线性关系（r=0．9998），平均回收率为100．26％，相对标准偏差为1．52％（n=6）。该法操作简便，分析速度快，结果准确，可用于感冒止咳颗粒中黄芩苷含量的测定。

A method for the determination of baicalin based on HPLC was developed. A mixture of nethanol, water and acetic acid （55 ： 45 ： 1,V/V/V） was used as the mobile phase. The linearity was in the ranges of 0.101-1.01 μg（r=0.9998）. The mean recovery was 100.26% with RSD of 1.52%（n= 6）. The method is simple, accurate, quickly and can be used for the determination of baicalin contant in Ganmao Zhike Granules.