流动注射分析光度法测定食品中Co(Ⅱ)

Determination of Cobalt in Food by FIow Injection Analysis Spectrophotometry

自行研制了一套流动比色装置，进行了双光束流动注射分析光度法的应用研究。基于二溴羟基苯荧光酮（DBHPF）与钴形成紫色络合物的原理，提出了一种新的流动注射微分测试技术，方法进样速度为110～120次／时，浓度在0～0．6μg／mL呈良好的线性关系。对0．3μg／mL的Co（Ⅱ）连续11次进样平行测定相对标准偏差为1．2％。用于食品中微量钴的测定，结果满意。

A new flow colorimetry device has been described for the determination of Co （ Ⅱ ） using dualbeam flow injection analysis spectrophotometry, based on the purple complex formed between dibromohydroxyphenyl fluorone and cobalt. The method showed good linearity in Co （ Ⅱ ） concentration range of 0-0.6 μg/mL, and the RSD for 0. 3 μg/mL Co （ Ⅱ ） was 1.2% （n= 11）. This method was used for the determination of cobalt in food with satisfactory result.