ET-1mRNA在弥漫性脑损伤中的表达

Expression of ET - 1 mRNA of brain after diffuse brain injury

目的探讨弥漫性脑损伤脑组织不同时间段ET-1mRNA表达。方法依据Marmarou’s弥漫性脑损伤动物模型有改进，应用SD雄性大鼠55只，随机分为两组：假手术（对照组）（n=5）和弥漫性脑损伤组（n=50），损伤组再按照不同时间段分组（各组n=5只），损伤后大鼠自由进食饮水，按0．5、1、3、6、12、24、48、72h、1周、2周等时间段处死大鼠，提取大鼠皮层脑组织，一部分脑组织应用realtimeRT—PCR检测对照组与不同时间段外伤组ET—1mRNA表达，另取一部分脑组织蘸干脑组织血迹，应用分析天平测定脑组织测得脑组织湿重，放入C101型电热恒温鼓风干燥箱105℃烘干至恒重，应用分析天平测定组织干重，应用脑组织干湿重比表示脑组织含水量。结果对照组与0．5、1、3、6、12、24、48、72h、1周、2周时间段外伤组Er—mRNACt值分别为：30．83：0．38，31．21±0．52，33．47±0．44，34.15±0．64，35.77±0．42，33.35±0.58，31．21±0．58，31.16±0、27，30.38±0.58，30．38±0．37，30.34±0.37。采用Kurskal—Wallis秩和检验，P〈0．01，对照组与不同时间组之间比较差异有统计学意义。结论弥漫性脑损伤后ET—1mRNA表达短期增加，6h就达到高峰。

Objective To determine the expression of ET - 1 mRNA in brain following diffuse brain injury. Methods Fifty - five male SD rats were randomly divided into two, groups： control group（ n = 5 ） , DBI group（ they were divided into ten groups again, n = 5）. Diffuse contusion and laceration of brain was produced by Marmarou＇ s brain injury model, Determine and evaluate the expression of ET - 1 mRNA at different time after brain iniury. Results The expression of ET - 1 mRNA upregulate rapidly after brain injury, it reached top at 6 hours, then declined slowly. The expression of ET - 1mRNA were different among groups by Karskal - Wallis test, P 〈 0.01. Conclusion The expression of ET - 1 mRNA following diffuse brain injury is upregulate rapidly in short time. It reaches the top at 6 hours.